Perfectly matched 20-nucleotide guide RNA sequences enable robust genome editing using high-fidelity SpCas9 nucleases. Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage. GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases. Prediction of off-target activities for the end-to-end design of CRISPR guide RNAs. DNA targeting specificity of RNA-guided Cas9 nucleases. CCTop: an intuitive, flexible and reliable CRISPR/Cas9 target prediction tool. Stemmer, M., Thumberger, T., Del Sol Keyer, M., Wittbrodt, J. CRISPRscan: designing highly efficient sgRNAs for CRISPR-Cas9 targeting in vivo. Optimized libraries for CRISPR-Cas9 genetic screens with multiple modalities. Deep learning improves prediction of CRISPR-Cpf1 guide RNA activity. Orthologous CRISPR-Cas9 enzymes for combinatorial genetic screens. Sequence determinants of improved CRISPR sgRNA design. WU-CRISPR: characteristics of functional guide RNAs for the CRISPR/Cas9 system. The Genotype-Tissue Expression (GTEx) project. Web-based design and analysis tools for CRISPR base editing. CRISPy-web: an online resource to design sgRNAs for CRISPR applications. Cas-Designer: a web-based tool for choice of CRISPR-Cas9 target sites. APPRIS: annotation of principal and alternative splice isoforms. The Consensus Coding Sequence (CCDS) project: identifying a common protein-coding gene set for the human and mouse genomes. Scalable design of paired CRISPR guide RNAs for genomic deletion. CRISPR library designer (CLD): software for multispecies design of single guide RNA libraries. FlashFry: a fast and flexible tool for large-scale CRISPR target design. CHOPCHOP v3: expanding the CRISPR web toolbox beyond genome editing. Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases. E-CRISP: fast CRISPR target site identification. GUIDES: sgRNA design for loss-of-function screens. Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. This study provides one of the first systematic comparisons of on- and off-target scoring algorithms, notable in part for its organization of external data.ĭoench, J. Evaluation of off-target and on-target scoring algorithms and integration into the guide RNA selection tool CRISPOR. This study demonstrated the importance of transcription start site annotation to the effective use of CRISPRa and CRISPRi technologies. Optimizing sgRNA position markedly improves the efficiency of CRISPR/dCas9-mediated transcriptional repression. Radzisheuskaya, A., Shlyueva, D., Müller, I. Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation. In vivo high-throughput profiling of CRISPR-Cpf1 activity. Engineered Cpf1 variants with altered PAM specificities. A ‘new lease of life’: FnCpf1 possesses DNA cleavage activity for genome editing in human cells. Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system. Engineered CRISPR-Cas9 nuclease with expanded targeting space. Engineered CRISPR-Cas9 nucleases with altered PAM specificities. ClinVar: public archive of relationships among sequence variation and human phenotype. RNA-programmed genome editing in human cells. RNA-guided human genome engineering via Cas9. Multiplex genome engineering using CRISPR/Cas systems. Genome-scale CRISPR-mediated control of gene repression and activation. Targeted genomic rearrangements using CRISPR/Cas technology. Search-and-replace genome editing without double-strand breaks or donor DNA. C in genomic DNA without DNA cleavage.This study developed ‘base editors’ by fusing both an APOBEC and a UGI domain to nickase Cas9, causing the deamination of target cytidines and thus their conversion to uracil, enabling site-specific editing in the absence of an exogenous repair template. Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage. These studies provide guidance on the optimization of experimental conditions and template design for homology-directed repair. Enhanced CRISPR/Cas9-mediated precise genome editing by improved design and delivery of gRNA, Cas9 nuclease, and donor DNA. Liang, X., Potter, J., Kumar, S., Ravinder, N. Optimization of scarless human stem cell genome editing. Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria. A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.
0 Comments
Leave a Reply. |